Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neurotransmitters and, upon binding to the IP3 receptor (IP3R), triggers the release of intracellular, luminal calcium to the cytosol. Several additional cellular forms of inositol phosphates have been discovered. These include some which have been recently discovered and characterized to possess up to seven or eight phosphate groups, including pyrophosphates (PP-IPs: pyrophosphate-inositol phosphates). It has been proposed that high-energy pyrophosphates may participate in protein phosphorylation.
Inositol Hexakisphosphate (IP6) Kinase, or PiUS (Pi Uptake Stimulator) has ATP synthase activity and transfers a phosphate from PP-IP5 to ADP to form ATP thus suggesting an important physiological role for PP-IP5 and IP6 kinase.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).Can be blocked with IP6 Kinase peptide (ab5007).
This antibody detects an ~50 kDa protein representing IP6 kinase from rat brain extract.
Inositol phosphokinase (IPK) family are responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4.