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Synthetic peptide corresponding to residues in Human ITPR1.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab108517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 314 kDa.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
Lane 3: Human brain tissue lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab108517 was shown to recognize IP3 receptor when IP3 receptor knockout samples were used, along with additional cross-reactive bands. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab108517 has not yet been referenced specifically in any publications.