製品の概要
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製品名Anti-IP10 antibody
IP10 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to IP10
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由来種Rabbit
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特異性This antibody does not cross-react with murine CXCL9 and CXCL11.
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アプリケーション適用あり: WB, ELISA, Neutralising, ICC/IFmore details
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種交差性交差種: Mouse
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免疫原
Highly pure (>98%) recombinant mIP-10 ( mouse Interferon Inducible Protein 10)
製品の特性
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製品の状態Lyophilised:Reconstitute with 200µl of sterile water.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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バッファーPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプunknown
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軽鎖の種類unknown
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab9938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| ELISA | Use at an assay dependent concentration. To detect mIP-10 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIP-10.Western Blot:To detect mIP-10 | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mIP-10 (15.00 ng/ml), a concentration of 0.5-0.9 µg/ml of this antibody is required. | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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機能Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
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配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
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翻訳後修飾CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 15945 Mouse
- SwissProt: P17515 Mouse
- Unigene: 877 Mouse
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別名
- Interferon gamma induced factor MOB1, mouse, homolog of antibody
- Interferon gamma induced protein 10 antibody
- 10 kDa interferon gamma induced protein antibody
see all
画像
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Western blot - Anti-IP10 antibody (ab9938)Western blot performed with ab9938 at 0.2 ug/ml
Lane 1: MultiMark MultiColor Standard (Invitrogen)
Lane 2: 250 ng Murine IP-10
Lane 3: 125 ng Murine IP-10
Lane 4: 62.5 ng Murine IP-10
Lane 5: 31.25 ng Murine IP-10
Lane 6: 15.625 ng Murine IP-10
Lane 7: 7.8 ng Murine IP-10
Lane 8: 3.9 ng Murine IP-10
Lane 9: 1.95 ng Murine IP-10
Lane 10: 0.975 ng Murine IP-10
Lane 11: 0.4875 ng Murine IP-10
Lane 12 0.24 ng Murine IP-10 -
Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody (ab9938)Staining of colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary. -
Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody (ab9938)Staining in colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.
参考文献
This product has been referenced in:
- Zhu X et al. A distinct brain pathway links viral RNA exposure to sickness behavior. Sci Rep 6:29885 (2016). Read more (PubMed: 27435819) »
- Chan CC et al. The role of interferon-? inducible protein-10 in a mouse model of acute liver injury post induced pluripotent stem cells transplantation. PLoS One 7:e50577 (2012). Neutralising ; Mouse . Read more (PubMed: 23227188) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"