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Our Abpromise guarantee covers the use of ab9807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration. To detect hIP-10 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIP-10.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIP-10 (100 ng/ml), a concentration of 2.0 - 3.0 µg/ml of this antibody is required.|
|WB||Use at an assay dependent concentration. To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|IHC-P||Use at an assay dependent concentration.|
ab9807 staining IP10 in Human carcinoid tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a pH 6.0 sodium citrate buffer. Samples were incubated with primary antibody (1ug/ml) overnight at 4°C. A HRP polymer detection system was used with a DAB chromogen.
ab9807 staining IP10 in Human skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody