製品の概要

  • 製品名Anti-IP10 antibody
    IP10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IP10
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human IP10.

    (Peptide available as ab47417.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal against Human recombinant IP10 full length protein

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 11 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻訳後修飾CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Interferon gamma induced factor MOB1, mouse, homolog of antibody
    • Interferon gamma induced protein 10 antibody
    • 10 kDa interferon gamma induced protein antibody
    • 10 kDa interferon gamma-induced protein antibody
    • C X C motif chemokine 10 antibody
    • C7 antibody
    • Chemokine (C X C motif) ligand 10 antibody
    • Chemokine CXC motif ligand 10 antibody
    • Crg 2 antibody
    • CRG2 antibody
    • CXCL10 antibody
    • CXCL10(1-73) antibody
    • CXL10_HUMAN antibody
    • Gamma IP10 antibody
    • Gamma-IP10 antibody
    • gIP 10 antibody
    • GIP10 antibody
    • IFI10 antibody
    • INP 10 antibody
    • INP10 antibody
    • Interferon activated gene 10 antibody
    • Interferon activated gene 10 antibody
    • Interferon gamma induced cell line antibody
    • Interferon inducible cytokine IP 10 antibody
    • Interferon inducible cytokine IP10 antibody
    • IP 10 antibody
    • IP-10 antibody
    • Mob 1 antibody
    • MOB1 antibody
    • Protein 10 from interferon (gamma) induced cell line antibody
    • SCYB10 antibody
    • Small inducible cytokine B10 antibody
    • Small inducible cytokine B10 precursor antibody
    • Small inducible cytokine subfamily B (Cys X Cys) member 10 antibody
    • Small inducible cytokine subfamily B CXC member 10 antibody
    • Small inducible cytokine subfamily B, member 10 antibody
    • Small-inducible cytokine B10 antibody
    see all

Anti-IP10 antibody 画像

  • Anti-IP10 antibody (ab47045) at 1 µg/ml + Recombinant human IP10 protein (ab9810) at 0.01 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • ab47045 staining human liver (healthy) sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed. Blocking of the sample was done with 5 minutes of peroxidase block and 10 minutes of protein block at 20°C, followed by staining with ab47045 at 1/50 for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit polyclonal antibody was used as the secondary antibody.

    See Abreview

Anti-IP10 antibody (ab47045) 使用論文

This product has been referenced in:
  • Ploquin MJ  et al. Elevated Basal Pre-infection CXCL10 in Plasma and in the Small Intestine after Infection Are Associated with More Rapid HIV/SIV Disease Onset. PLoS Pathog 12:e1005774 (2016). IHC-Fr ; Cynomolgus Monkey . Read more (PubMed: 27509048) »
  • Chmielewski S  et al. STAT1-dependent signal integration between IFN? and TLR4 in vascular cells reflect pro-atherogenic responses in human atherosclerosis. PLoS One 9:e113318 (2014). IHC-P ; Human . Read more (PubMed: 25478796) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal Liver)
Specification Normal Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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投稿 Sep 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"