Anti-Ionotropic Glutamate receptor 2 抗体 [EP966Y] (ab52932)

製品の概要

  • 製品名Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y]
    Ionotropic Glutamate receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP966Y] to Ionotropic Glutamate receptor 2
  • アプリケーション適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC/IF,IHC-Fr or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminus of human GluR2.

  • ポジティブ・コントロール
    • Human brain cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
IP 1/40.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC/IF,IHC-Fr or IHC-P.
  • ターゲット情報

    • 機能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
    • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
    • 翻訳後修飾Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AMPA 2 antibody
      • AMPA selective glutamate receptor 2 antibody
      • AMPA-selective glutamate receptor 2 antibody
      • AMPA2 antibody
      • GluA2 antibody
      • GLUR 2 antibody
      • GLUR B antibody
      • GluR K2 antibody
      • GluR-2 antibody
      • GluR-B antibody
      • GluR-K2 antibody
      • GLUR2 antibody
      • GLURB antibody
      • Glutamate receptor 2 antibody
      • Glutamate receptor ionotropic AMPA 2 antibody
      • Glutamate receptor ionotropic antibody
      • Gria2 antibody
      • GRIA2_HUMAN antibody
      • HBGR2 antibody
      see all

    Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] 画像

    • Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/2000 dilution + Human brain cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 99 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/500 dilution

      Lane 1 : Human Brain
      Lane 2 : Rat Brain
      Lane 3 : Mouse Brain


      Predicted band size : 99 kDa

      Secondary antibody - anti-rabbit HRP (ab6721)

    Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) 使用論文

    This product has been referenced in:
    • Chen C  et al. Epigenetic modification of PKM? rescues aging-related cognitive impairment. Sci Rep 6:22096 (2016). WB ; Rat . Read more (PubMed: 26926225) »
    • Gascon E  et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med 20:1444-51 (2014). WB, IHC ; Mouse . Read more (PubMed: 25401692) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
    Sample Rat Tissue lysate - whole (hippocampi total membrane fraction)
    Specification hippocampi total membrane fraction
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Feb 25 2014

    Thanks for your enquiry. The sequence homology between the immunogen sequence of ab52932 and human Glutamate receptors are as follows. human GluR1: 44.4% human GluR3: 61.5% human GluR4: 56.2% Based on sequence analysis, ab52932 is most like...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"