Anti-Ionotropic Glutamate receptor 2 抗体 (ab20673)

製品の概要

  • 製品名Anti-Ionotropic Glutamate receptor 2 antibody
    Ionotropic Glutamate receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ionotropic Glutamate receptor 2
  • アプリケーション適用あり: IHC-FoFr, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Chicken, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2 .

    (Peptide available as ab25708.)

  • ポジティブ・コントロール
    • ab20673 gave a positive result in the following tissue lysates: Mouse brain Mouse cortex Rat brain

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab20673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/300.
WB Use a concentration of 3 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).Can be blocked with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708).

ターゲット情報

  • 機能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
  • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
  • 翻訳後修飾Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AMPA 2 antibody
    • AMPA selective glutamate receptor 2 antibody
    • AMPA-selective glutamate receptor 2 antibody
    • AMPA2 antibody
    • GluA2 antibody
    • GLUR 2 antibody
    • GLUR B antibody
    • GluR K2 antibody
    • GluR-2 antibody
    • GluR-B antibody
    • GluR-K2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate receptor 2 antibody
    • Glutamate receptor ionotropic AMPA 2 antibody
    • Glutamate receptor ionotropic antibody
    • Gria2 antibody
    • GRIA2_HUMAN antibody
    • HBGR2 antibody
    see all

Anti-Ionotropic Glutamate receptor 2 antibody 画像

  • Lane 1 : Marker
    Lanes 2 - 5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
    Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
    Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
    Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml

    Secondary
    Lanes 2 - 5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution


    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa.
  • Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

    The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.

  • Immunohistochemistical detection of Ionotropic Glutamate receptor 2 using antibody (ab20673) on PFA perfusion-fixed frozen rat brain sections. Primary antibody was used at 1/300, incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The image demonstrates immunostaining obtained in the rat cortex, thin and punctuated cytoplasmic staining is observed as previously described for the hippocampus (J neurosci 2003 Nov 1923(33):10521-30). There is some staining in cellular processes as well (arrows).

    See Abreview

Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) 使用論文

This product has been referenced in:
  • Tada H  et al. Estrous Cycle-Dependent Phasic Changes in the Stoichiometry of Hippocampal Synaptic AMPA Receptors in Rats. PLoS One 10:e0131359 (2015). Read more (PubMed: 26121335) »
  • Qiao H  et al. 14-3-3 proteins are required for hippocampal long-term potentiation and associative learning and memory. J Neurosci 34:4801-8 (2014). Read more (PubMed: 24695700) »

See all 4 Publications for this product

Product Wall

Hello and thank you for contacting us. Yes, the concentration of ab20673 is 1.0 mg/ml and we will update our website. Please let me know if you have further questions.

Thank you for contacting us.

The ab20673has not been tested on human samples yet, but is thought to be working as the homology is high. Please let me know if you would like to test this on your own asI could offer you our AbTrial discount.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Apr 13 2010

Thanks for your enquiry. The sequence homology between the immunogen sequence of ab20673 and human Glutamate receptors are as follows. human GluR1: 60% human GluR2: 92% human GluR3: 30% human GluR4: 40% Based on sequence analysis, ab20673 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"