製品の概要

  • 製品名
    Anti-Involucrin antibody [SY8]
    Involucrin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SY8] to Involucrin
  • 特異性
    Keratinocytes of epidermis and other stratified squamous epithelia
  • アプリケーション
    適用あり: IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Human, Pig, Gorilla
  • 免疫原

    Pure involucrin from human keratinocytes.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    SY8
  • ミエローマ
    P3x63
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • 組織特異性
    Keratinocytes of epidermis and other stratified squamous epithelia.
  • 配列類似性
    Belongs to the involucrin family.
  • 翻訳後修飾
    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
  • 細胞内局在
    Cytoplasm. Constituent of the scaffolding of the cornified envelope.
  • Information by UniProt
  • 参照データベース
  • 別名
    • INVO_HUMAN antibody
    • Involucrin antibody
    • IVL antibody

Anti-Involucrin antibody [SY8] 画像

  • Ab20202 staining Human skin Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Involucrin antibody [SY8] (ab20202) 使用論文

This product has been referenced in:
  • Buerger C  et al. Inflammation dependent mTORC1 signaling interferes with the switch from keratinocyte proliferation to differentiation. PLoS One 12:e0180853 (2017). Human . Read more (PubMed: 28700632) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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