Anti-Involucrin 抗体 (ab53112)
Key features and details
- Rabbit polyclonal to Involucrin
- Suitable for: ICC/IF, ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Involucrin antibody
Involucrin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Involucrin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, ELISA, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
A synthetic peptide derived from human Involucrin.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab53112 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53112の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration. PubMed: 21067603
|
|
ELISA |
Use at an assay dependent concentration.
|
|
WB |
1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
|
|
IHC-P | (5) |
Use at an assay dependent concentration.
|
特記事項 |
---|
ICC/IF
Use at an assay dependent concentration. PubMed: 21067603 |
ELISA
Use at an assay dependent concentration. |
WB
1/300 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
組織特異性
Keratinocytes of epidermis and other stratified squamous epithelia. -
配列類似性
Belongs to the involucrin family. -
翻訳後修飾
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
細胞内局在
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
-
参照データベース
- Entrez Gene: 3713 Human
- Omim: 147360 Human
- SwissProt: P07476 Human
- Unigene: 516439 Human
-
別名
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
画像
-
ab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
-
All lanes : Anti-Involucrin antibody (ab53112) at 1/300 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunising peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa -
ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (41)
ab53112 は 41 報の論文で使用されています。
- Ogami T et al. MAB21L4 regulates the TGF-β-induced expression of target genes in epidermal keratinocytes. J Biochem 171:399-410 (2022). PubMed: 34908107
- Erratico S et al. Effective high-throughput isolation of enriched platelets and circulating pro-angiogenic cells to accelerate skin-wound healing. Cell Mol Life Sci 79:259 (2022). PubMed: 35474498
- Ali G & Abdelalim EM Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells. STAR Protoc 3:101613 (2022). PubMed: 35990735
- Feng D et al. CBP-mediated Wnt3a/ß-catenin signaling promotes cervical oncogenesis initiated by Piwil2. Neoplasia 23:1-11 (2021). PubMed: 33190089
- Charruyer A et al. Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis. Aging Cell 20:e13310 (2021). PubMed: 33524216