The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Keratinocytes of epidermis and other stratified squamous epithelia.
Belongs to the involucrin family.
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Cytoplasm. Constituent of the scaffolding of the cornified envelope.
Melo SP et al. Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant. Mol Ther22:725-33 (2014).
Read more (PubMed: 24390279) »
Lamb R & Ambler CA Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model. PLoS One8:e52494 (2013).
Read more (PubMed: 23326335) »