INTS5 is a subunit of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
All lanes : Anti-INTS5 antibody (ab74405) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 108 kDa Observed band size : 108 kDa Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Immunoprecipitation - INTS5 antibody (ab74405)
Detection of INTS5 by Western Blot of Immunprecipitate.
ab74405, at 1µg/ml, staining INTS5 in HeLa whole cell lysate immunoprecipitated using ab74405 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.