Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Anti-Intra Acrosomal Protein antibody 画像
Western blot - Intra Acrosomal Protein antibody (ab81229)
Anti-Intra Acrosomal Protein antibody (ab81229) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
29 kDaObserved band size :
33 kDa (why is the actual band size different from the predicted?
Anti-Intra Acrosomal Protein antibody (ab81229) 使用論文
has not yet been referenced specifically in any publications.
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