製品の概要

  • 製品名
    Anti-intestinal FABP antibody
    intestinal FABP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to intestinal FABP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Horse, Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human intestinal FABP.

    (Peptide available as ab88236.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Caco2; SW480; LoVo.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).

ターゲット情報

  • 機能
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • 組織特異性
    Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • 配列類似性
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • ドメイン
    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FABP 2 antibody
    • FABP2 antibody
    • FABPI antibody
    • FABPI_HUMAN antibody
    • Fatty acid binding protein 2 intestinal antibody
    • Fatty acid binding protein antibody
    • Fatty acid binding protein intestinal antibody
    • Fatty acid-binding protein 2 antibody
    • Fatty acid-binding protein antibody
    • I FABP antibody
    • I-FABP antibody
    • IFABP antibody
    • intestinal antibody
    • Intestinal fatty acid binding protein 2 antibody
    • Intestinal-type fatty acid-binding protein antibody
    • MGC133132 antibody
    • OTTHUMP00000163925 antibody
    see all

画像

  • All lanes : Anti-intestinal FABP antibody (ab77386) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 115 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

プロトコール

参考文献

ab77386 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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