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Our Abpromise guarantee covers the use of ab60272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. Antibody detection limit dilution 1:64000.|
|WB||Use a concentration of 0.001 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human intestinal FABP peptide (ab115238).|
|IHC-P||Use a concentration of 2 - 3 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23236492|
ab60272 staining FABP in Human small intestine by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue sections underwent antigen retrieval by steam with citrate buffer pH 6.0. The AP staining procedure was used for detection.