Anti-Intersectin 1 抗体 (ab118262)
製品の概要
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製品名Anti-Intersectin 1 antibody
Intersectin 1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Intersectin 1
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由来種Rabbit
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment, corresponding to amino acids 646-840 of Human Intersectin 1 (BC116186).
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ポジティブ･コントロール
- Human fetal brain lysate
法規制情報
製品の特性
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製品の状態Lyophilised:Reconstitute in 200µl Steriled Distilled Water
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab118262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 195 kDa. |
ターゲット情報
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機能Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1.
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組織特異性Isoform 2 is ubiquitous in adult and fetal tissues with high expression in skeletal muscle, heart, spleen, ovary, testis and all fetal tissues tested and low expression in thymus, blood, lung, liver and pancreas. Isoform 1 is expressed almost exclusively in the brain, in all brain regions. Not expressed in the spinal cord.
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配列類似性Contains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 2 EF-hand domains.
Contains 2 EH domains.
Contains 1 PH domain.
Contains 5 SH3 domains. -
ドメインSH3-3, SH3-4 and SH3-5, but not SH3-1 and SH3-2 domains, bind to dynamin (By similarity). SH3-1 and SH3-4 bind to ARHGAP31.
The KLERQ domain binds to SNAP-25 and SNAP-23. -
細胞内局在Endomembrane system. Cell junction > synapse > synaptosome. Cell projection > lamellipodium.
- Information by UniProt
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参照データベース
- Entrez Gene: 6453 Human
- Entrez Gene: 16443 Mouse
- Entrez Gene: 29491 Rat
- Omim: 602442 Human
- SwissProt: Q15811 Human
- SwissProt: Q9Z0R4 Mouse
- SwissProt: Q9WVE9 Rat
- Unigene: 160324 Human
see all -
別名
- Human intersectin SH3 domain containing protein SH3P17 antibody
- Intersectin 1 SH3 domain protein antibody
- intersectin 1 short form variant 3 antibody
see all
画像
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Western blot - Anti-Intersectin 1 antibody (ab118262)Anti-Intersectin 1 antibody (ab118262) at 1/500 dilution + Human fetal brain lysate
Predicted band size: 195 kDa
Additional bands at: 129 kDa (possible isoform), 138 kDa (possible isoform)
プロトコール
参考文献
This product has been referenced in:
- Humphries AC et al. Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. J Cell Sci 127:673-85 (2014). Read more (PubMed: 24284073) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"