製品の概要

  • 製品名Anti-Interleukin 34 antibody
    Interleukin 34 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Interleukin 34
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Interleukin 34.

    (Peptide available as ab94831.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Caco 2; PC3; HepG2

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent dilution. Detects a band of approximately 48 kDa (predicted molecular weight: 27 kDa).

ターゲット情報

  • 機能Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.
  • 組織特異性Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.
  • 配列類似性Belongs to the IL-34 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C16orf77 antibody
    • Chromosome 16 open reading frame 77 antibody
    • FPT025 antibody
    • IL 34 antibody
    • IL-34 antibody
    • Il34 antibody
    • IL34_HUMAN antibody
    • Interleukin 34 antibody
    • Interleukin-34 antibody
    • Interleukin34 antibody
    • MGC34647 antibody
    see all

Anti-Interleukin 34 antibody 画像

  • All lanes : Anti-Interleukin 34 antibody (ab75723) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : PC3 (human prostate carcinoma cell line) Whole cell lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • ICC/IF image of ab75723 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75723, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Interleukin 34 antibody (ab75723) 使用論文

This product has been referenced in:
  • Chemel M  et al. Interleukin 34 expression is associated with synovitis severity in rheumatoid arthritis patients. Ann Rheum Dis : (2011). IHC ; Human . Read more (PubMed: 22039170) »

See 1 Publication for this product

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