製品の概要

  • 製品名Anti-Interleukin 34 antibody [1D12]
    Interleukin 34 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1D12] to Interleukin 34
  • アプリケーション適用あり: WB, ICC/IF, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 21-242 of Human Interleukin 34

  • ポジティブ・コントロール
    • Interleukin 34-hIgGFc transfected HEK293 cell lysate; U251 cells; K562 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 27 kDa.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.
Flow Cyt 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.
  • 組織特異性Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.
  • 配列類似性Belongs to the IL-34 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C16orf77 antibody
    • Chromosome 16 open reading frame 77 antibody
    • FPT025 antibody
    • IL 34 antibody
    • IL-34 antibody
    • Il34 antibody
    • IL34_HUMAN antibody
    • Interleukin 34 antibody
    • Interleukin-34 antibody
    • Interleukin34 antibody
    • MGC34647 antibody
    see all

Anti-Interleukin 34 antibody [1D12] 画像

  • Anti-Interleukin 34 antibody [1D12] (ab101443) at 1/500 dilution + Interleukin 34-hIgGFc transfected HEK293 cell lysate

    Predicted band size : 27 kDa
  • ab101443, at 1/200 dilution, staining Interleukin 34 in U251 cells by Immunofluorescence analysis (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ab101443 staining Interleukin 34 in K562 cells (green) at 1/200 by Flow cytometry. Negative control (purple).

Anti-Interleukin 34 antibody [1D12] (ab101443) 使用論文

This product has been referenced in:
  • Baghdadi M  et al. Chemotherapy-Induced IL34 Enhances Immunosuppression by Tumor-Associated Macrophages and Mediates Survival of Chemoresistant Lung Cancer Cells. Cancer Res 76:6030-6042 (2016). Read more (PubMed: 27550451) »
  • Wang Y  et al. IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia. Nat Immunol 13:753-60 (2012). Read more (PubMed: 22729249) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer 6.0
Sample Rhesus monkey Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Ms. Lindsey Gerngross

Verified customer

投稿 Oct 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"