Anti-Interferon gamma 抗体 [MD-1] (ab25014)

製品の概要

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab25014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Use at an assay dependent dilution.

ELISpot Use at an assay dependent concentration.

Use at an assay dependent dilution.

Neutralising Use at an assay dependent concentration.

Antibody activity is 15,000 neutralizing units/mg protein. One neutralizing unit is defined as the total amount of antibodies sufficient for neutralizing one antiviral unit of human IFN-gamma as calibrated against NIH standard Gg 23-901-530.

WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.

Use at an assay dependent dilution.

Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Interferon gamma (ab25101). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab25101 as Detection.
Flow Cyt Use at an assay dependent concentration.

Good for intracellular flow cytometric staining. Use at an assay dependent dilution.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Use at an assay dependent dilution.

ターゲット情報

  • 機能Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 組織特異性Released primarily from activated T lymphocytes.
  • 関連疾患In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • 配列類似性Belongs to the type II (or gamma) interferon family.
  • 翻訳後修飾Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IFG antibody
    • IFI antibody
    • IFN gamma antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    see all

Anti-Interferon gamma antibody [MD-1] 画像

  • Standard curve for Interferon gamma (Analyte: ab51240); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab25014 at 5µg/ml and Detector Antibody ab25101 at 0.5µg/ml.

プロトコール

Anti-Interferon gamma antibody [MD-1] (ab25014) 使用論文

ab25014 has not yet been referenced specifically in any publications.

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Thank you for contacting us. We can certainly provide these in 500ug or 1mg size as a special request. Please let me know if you would like to place an order. I hope this information is helpful to you. Please do not hesitate to contact u...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Purified protein (Plasma)
Specification Plasma
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Type Direct
Username

Ms. Angel Morrow

Verified customer

投稿 Jul 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"