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Recombinant human IFN gamma derived from E.coli.
Our Abpromise guarantee covers the use of ab25101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ELISpot||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration.
In vitro neutralization. One Neutralizing Unit is defined as the total amount of antibodies sufficient for neutralizing one laboratory unit of recombinant Human IFNG (1 Unit = ~50 pg of pure Human IFNG). Activity ≥ 10^4 Neutralizing U/mg protein.
|ICC||Use at an assay dependent concentration.
Follow an intracellular staining protocol.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MD-1] to Interferon gamma (ab25014).
For sandwich ELISA, use this antibody as detection at 0.5µg/ml with ab25014 as capture.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab25101 staining Human tonsil. Staining is localised to the cytoplasm and protein is secreted.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be
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