Anti-Interferon beta 抗体 (ab85803)

製品の概要

  • 製品名
    Anti-Interferon beta antibody
    Interferon beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Interferon beta
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Macaque monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Interferon beta.

    (Peptide available as ab95054.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2 and WI38 whole cell lysates and in the following human tissue lysates: Liver; Lung; Kidney; Spleen.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

画像

  • All lanes : Anti-Interferon beta antibody (ab85803) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : WI-38 whole cell lysate (ab3960)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Lung (Human) Tissue Lysate
    Lane 5 : Human kidney tissue lysate - total protein (ab30203)
    Lane 6 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,120 kDa,160 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab85803 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab85803 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Specification
hepatocytes
Treatment
20 ug/ml poly(I:C)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Sep 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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