Anti-Interferon beta 抗体 (ab85803)

製品の概要

  • 製品名Anti-Interferon beta antibody
    Interferon beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Interferon beta
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Interferon beta.

    (Peptide available as ab95054.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2 and WI38 whole cell lysates and in the following human tissue lysates: Liver; Lung; Kidney; Spleen.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

Anti-Interferon beta antibody 画像

  • All lanes : Anti-Interferon beta antibody (ab85803) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : WI-38 whole cell lysate (ab3960)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Lung (Human) Tissue Lysate
    Lane 5 : Human kidney tissue lysate - total protein (ab30203)
    Lane 6 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,120 kDa,160 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab85803 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Interferon beta antibody (ab85803) 使用論文

ab85803 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment 20 ug/ml poly(I:C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Sep 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"