Anti-Interferon alpha 2b 抗体 [4E10] (HRP) (ab5258)

製品の概要

  • 製品名
    Anti-Interferon alpha 2b antibody [4E10] (HRP)
    Interferon alpha 2b 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4E10] to Interferon alpha 2b (HRP)
  • 標識
    HRP
  • 特異性
    Recognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
  • 特記事項

    Recommended ELISA procedure for human IFN alpha 2b determination: 1. Use 96-well Nunc Maxisorp plates for the assay. Coat microwells with 100 µl/well of antibody 9D3 (ab9386) diluted to 10 µg/ml in coating buffer (0.05 M Na-carbonate, pH 9.5). Incubate overnight at +4°C. 2. Aspirate wells and block with 150 µl/well of blocking buffer (2% BSA in PBS). Incubate at RT for 1 h. 3. Aspirate wells and wash 3 times with PBST (0.1% Tween-20 in PBS). 4. Prepare standard and sample dilutions in PBST. Recommended IFN alpha 2b concentrations: from 100 pg/ml to 100 ng/ml. Add 100 µl of each standard and sample into appropriate wells. Incubate at RT for 2 h. 5. Aspirate and wash 5 times with PBST. 6. Dilute detection antibody 4E10-HRP (ab5258) 1:1000 in PBST. Add 100 µl of diluted detection antibody to each well. Incubate at RT for 1 h. 7. Aspirate and wash 10 times with PBST. 8. Add 100 µl of substrate solution (TMB) to each well. Incubate at RT for 10-20 min. 9. Add 50 µl of stop solution (2 N H2SO4) to each well. Read absorbance at 450 nm.

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR308991-1 and GR308991-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Sandwich ELISA: 1/1000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Comments:
    This antibody can be used in two-site ELISA for the determination of human IFN-a2b.
    It recognizes a different epitope than monoclonal antibody 9D3 (ab9386).
    We recommend using this antibody (ab5258) as a detection antibody.
  • ターゲット情報

    • 機能
      Produced by macrophages, IFN-alpha have antiviral activities.
    • 配列類似性
      Belongs to the alpha/beta interferon family.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha 2a interferon antibody
      • IFN alpha 2b antibody
      • IFN-alpha-2 antibody
      • IFNA antibody
      • Ifna2 antibody
      • IFNA2_HUMAN antibody
      • INFA2 antibody
      • Interferon alpha 2 antibody
      • Interferon alpha A antibody
      • Interferon alpha-2 antibody
      • Interferon alpha-A antibody
      • LeIF A antibody
      • LeIFA antibody
      see all

    プロトコール

    参考文献

    ab5258 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Recombinant protein (E coli)
    Loading amount
    2 µg
    Specification
    E coli
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 13 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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