Anti-Interferon alpha 2b 抗体 [4E10] (ab9388)

製品の概要

  • 製品名Anti-Interferon alpha 2b antibody [4E10]
    Interferon alpha 2b 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4E10] to Interferon alpha 2b
  • 特異性Recognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g. Does not inhibit antiviral activity of IFN-a2b in vitro.
  • アプリケーション適用あり: Indirect ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
  • 特記事項The neutralising bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the test antibodies for 2 h at RT and then added to the cell monolayer. 24 h later the cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. Polyclonal antibodies to respective interferons and irrelevant monoclonal antibody of the same isotype were used as controls.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名4E10
  • ミエローマNS0/1
  • アイソタイプIgG1
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA 1/500 - 1/2000.
WB 1/500.

ターゲット情報

  • 機能Produced by macrophages, IFN-alpha have antiviral activities.
  • 配列類似性Belongs to the alpha/beta interferon family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha 2a interferon antibody
    • IFN alpha 2b antibody
    • IFN-alpha-2 antibody
    • IFNA antibody
    • Ifna2 antibody
    • IFNA2_HUMAN antibody
    • INFA2 antibody
    • Interferon alpha 2 antibody
    • Interferon alpha A antibody
    • Interferon alpha-2 antibody
    • Interferon alpha-A antibody
    • LeIF A antibody
    • LeIFA antibody
    see all

Anti-Interferon alpha 2b antibody [4E10] 画像

  • Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4 µg/ml).
    Lane 1 - interferon alpha 2b
    Lane 2 - prestained standard proteins.

Anti-Interferon alpha 2b antibody [4E10] (ab9388) 使用論文

ab9388 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Yes, these are the same lot numbers in all cases. The number after the - just indicates the time it was aliquotted, so if the GRXXXX is the same but the dash is different you can be assured the lot is still the same. Please...

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Thank you for contacting us.

We have used this antibody under reducing and denaturing conditions in western blot. We unfortunately haven't done any comparison studies between native vs reducing conditions. I am sorry we are unable to comment o...

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Thank you for contacting us. Concentrations of the antibodies ab9386 and ab9388 are determined spectrophotometrically by an adsorbance measurement at 280 nm. The absorbance value (A280) is divided by the extiction coefficient of mouse IgG - 1.35. I hop...

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Thank you for contacting Abcam. For both ab9386 and ab9388, we currently sell them in 100ul aliquots, at a concentration of 2mg/ml. From what I can see, there are no plans to change this concentration for future lots of the antibody and since it is a m...

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Thank you for your enquiry. We currently do not have an antibody to human interferon alpha that is biotinylated. We have the following human interferon alpha antibodies: ab9660, ab9386, ab8317, ab9388, and ab5258 (HRP conjugated). If you have any more ...

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To use monoclonal antibodies 9D3 and 4E10 in sandwich ELISA for the detection of huIFN-alpha-2b, 4E10 antibody should be labeled either with horse-radish peroxidase or biotin. Monoclonal antibody 9D3 should be used as a capture antibody (for plate coat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"