Anti-Integrin linked ILK 抗体 [EP1593Y] (ab52480)

製品の概要

  • 製品名Anti-Integrin linked ILK antibody [EP1593Y]
    Integrin linked ILK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1593Y] to Integrin linked ILK
  • 特異性This antibody reacts with Integrin linked ILK
  • アプリケーション適用あり: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, African Green Monkey
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Integrin linked ILK aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13418

  • ポジティブ・コントロール
    • Human heart muscle tissue and COS cell lysate
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP1593Y
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • 組織特異性Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.
  • ドメインA PH-like domain is involved in phosphatidylinositol phosphate binding.
  • 翻訳後修飾Autophosphorylated on serine residues.
  • 細胞内局在Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

Anti-Integrin linked ILK antibody [EP1593Y] 画像



  • Predicted band size : 51 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Integrin linked ILK knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg) 
    Lanes 1 - 4: Merged signal (red and green). Green - ab52480 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab52480 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. ab52480 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Overlay histogram showing HEK293 cells stained with ab52480 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52480, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution + COS-1 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • Immunohistochemical analysis of paraffin-embedded human heart muscle using ab52480 at a 1/50 dilution.
  • All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution

    Lane 1 : Rat Brain
    Lane 2 : Rat Heart
    Lane 3 : Rat Kidney
    Lane 4 : Rat Spleen


    Predicted band size : 51 kDa


    Exposure time : 3 minutes
  • Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) + PC12

    Predicted band size : 51 kDa

Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) 使用論文

This product has been referenced in:
  • Xing Y  et al. Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-ß2. Int J Mol Med N/A:N/A (2016). WB ; Human . Read more (PubMed: 27315599) »
  • Hoshino A  et al. Tumour exosome integrins determine organotropic metastasis. Nature 527:329-35 (2015). Human . Read more (PubMed: 26524530) »

See all 6 Publications for this product

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