Anti-Integrin linked ILK 抗体 [EP1593Y] (ab52480)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1593Y] to Integrin linked ILK
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Rat, Human, African green monkey
Related conjugates and formulations
製品の概要
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製品名
Anti-Integrin linked ILK antibody [EP1593Y]
Integrin linked ILK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1593Y] to Integrin linked ILK -
由来種
Rabbit -
特異性
This antibody reacts with Integrin linked ILK. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Rat, Human, African green monkey
交差が予測される動物種: Mouse, Cow -
免疫原
Synthetic peptide within Human Integrin linked ILK aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q13418 -
ポジティブ・コントロール
- Human heart muscle tissue and COS cell lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1593Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52480の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
For unpurified use at 1/2000 - 1/10000 dilution. |
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IP |
1/40.
For unpurified use at 1/30 dilution. |
|
IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. |
特記事項 |
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WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). For unpurified use at 1/2000 - 1/10000 dilution. |
IP
1/40. For unpurified use at 1/30 dilution. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. |
ターゲット情報
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機能
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. -
組織特異性
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain. -
ドメイン
A PH-like domain is involved in phosphatidylinositol phosphate binding. -
翻訳後修飾
Autophosphorylated on serine residues. -
細胞内局在
Cell junction > focal adhesion. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 540207 Cow
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q3SWY2 Cow
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
see all -
別名
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Integrin linked ILK knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab52480 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.Unpurified ab52480 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. Unpurified ab52480 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling Integrin linked ILK with Purified ab52480 at 1/100 dilution (7.13 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0).
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All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates with 5% NFDM/TBST
Lane 3 : Mouse heart lysates with 5% NFDM/TBST
Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 51 kDa
Observed band size: 51 kDa -
ab52480 (purified) at 1:40 dilution (2µg) immunoprecipitating Integrin linked ILK in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab52480 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52480 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/1000 dilution (Purified) + COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg with 5% NFDM/TBST
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution (unpurified) + COS-1 (African green monkey kidney fibroblast-like) cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) (unpurified) + PC12
Predicted band size: 51 kDa -
Immunohistochemical analysis of paraffin-embedded human heart muscle using unpurified ab52480 at a 1/50 dilution.
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All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution (unpurified)
Lane 1 : Rat Brain
Lane 2 : Rat Heart
Lane 3 : Rat Kidney
Lane 4 : Rat Spleen
Predicted band size: 51 kDa
Exposure time: 3 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab52480 は 18 報の論文で使用されています。
- Beetham H et al. Loss of Integrin-Linked Kinase Sensitizes Breast Cancer to SRC Inhibitors. Cancer Res 82:632-647 (2022). PubMed: 34921014
- Yu Y et al. Synergistic effect of Tripterygium glycosides and cisplatin on drug-resistant human epithelial ovarian cancer via ILK/GSK3β/Slug signal pathway. Am J Transl Res 14:2051-2062 (2022). PubMed: 35422913
- Chen H et al. GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathway. Bioengineered 13:10944-10955 (2022). PubMed: 35477364
- Yue Y et al. Interleukin-10 Deficiency Alters Endothelial Progenitor Cell-Derived Exosome Reparative Effect on Myocardial Repair via Integrin-Linked Kinase Enrichment. Circ Res 126:315-329 (2020). PubMed: 31815595
- Chen Q et al. Integrin-linked kinase improves uterine receptivity formation by activating Wnt/ß-catenin signaling and up-regulating MMP-3/9 expression. Am J Transl Res 12:3011-3022 (2020). PubMed: 32655826