製品の概要

  • 製品名
    Anti-Integrin beta 8 antibody
    Integrin beta 8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Integrin beta 8
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: CECTDPRSIG RFCEHCPTCY TACKENWNCM QCLHPHNLSQ AILDQCKTSC , corresponding to C terminal amino acids 611-660 of Human Integrin beta 8 (accession number: NP_002205)

  • ポジティブ・コントロール
    • Human placenta membrane lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituents: 2% Sucrose, 98% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Only Peptide ELISA tested

IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • IHC image of Integrin beta 8 staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80673, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-Integrin beta 8 antibody (ab80673) at 1 µg/ml + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Observed band size : 81 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Bian Q  et al. Mechanosignaling activation of TGFß maintains intervertebral disc homeostasis. Bone Res 5:17008 (2017). IHC-P ; Mouse . Read more (PubMed: 28392965) »
  • Mertens-Walker I  et al. The tumour-promoting receptor tyrosine kinase, EphB4, regulates expression of integrin-ß8 in prostate cancer cells. BMC Cancer 15:164 (2015). WB . Read more (PubMed: 25886373) »

See all 2 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Glioma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
Permeabilization
No
Specification
Glioma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Jun 11 2015

Application
Western blot
Loading amount
400000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Cell lysate - whole cell (primary astrocytes)
Specification
primary astrocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Dec 22 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Rat Cell (primary brain astrocytes)
Specification
primary brain astrocytes
Permeabilization
Yes - 0,1% Triton
Fixative
Paraformaldehyde
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投稿 Dec 19 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (Glioma)
Specification
Glioma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

投稿 Dec 18 2013

Unfortunately this antibody has not yet been tested in Flow Cytometry, so we don't have any data stating whether or not this antibody would be suitable for this application.

I am very pleased to hear you would like to accept our offer and test ab80673 in WB in mouse. This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview for WB in mouse and include this co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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