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RabMAb

Anti-Integrin beta 5 抗体 [EPR19170] (ab184312)

製品の概要

  • 製品名
    Anti-Integrin beta 5 antibody [EPR19170]
    Integrin beta 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR19170] to Integrin beta 5
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment within Mouse Integrin beta 5 aa 400-700. The exact sequence is proprietary.
    Database link: O70309

  • ポジティブ・コントロール
    • WB: Rat and mouse lung lysates; A549, Neuro-2a, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR19170
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab184312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).

ターゲット情報

画像

  • All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : Rat lung lysate at 20 µg
    Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
    Lane 4 : Mouse lung lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes; Lane 3: 1 minute; Lane 4: 3 minutes.

     

  • All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2 and 3: 15 seconds; Lane 4: 1 minute

プロトコール

参考文献

ab184312 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab184312.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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