製品の概要

  • 製品名Anti-Integrin beta 5 antibody
    Integrin beta 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Integrin beta 5
  • アプリケーション適用あり: ICC/IF, IHC-Fr, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Hamster
  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.

  • ポジティブ・コントロール
    • Small intestine, bone marrow and colon carcinoma HeLa Cells Rat gastric epithelial

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/20.
IHC-Fr Use at an assay dependent concentration. PubMed: 22025551
IP Use at an assay dependent concentration. PubMed: 22025551
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

Anti-Integrin beta 5 antibody 画像

  • Human small intestine (ab29276) stained with anti-Integrin beta 5 antibody, ab15459 by Immunohistochemistry (FFPE-sections).

  • ICC/IF image of ab15459 stained HeLa cells (ab150035ab15459), 1µg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15459 staining Integrin beta 5 in Rat gastric epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 0.25% serum free protein for 20 minutes at 28°C. Samples were incubated with primary antibody (1/50 in antibody diluent) for 2 hours at 28°C. An FITC-conjugated Goat anti-IgG polyclonal (1/80) was used as the secondary antibody. Nuclei were counterstained with propidium iodide (ab14083).

    See Abreview

Anti-Integrin beta 5 antibody (ab15459) 使用論文

This product has been referenced in:
  • Sarrazy V  et al. Integrins avß5 and avß3 promote latent TGF-ß1 activation by human cardiac fibroblast contraction. Cardiovasc Res 102:407-17 (2014). Read more (PubMed: 24639195) »
  • Klingberg F  et al. Prestress in the extracellular matrix sensitizes latent TGF-ß1 for activation. J Cell Biol 207:283-97 (2014). ICC/IF ; Human . Read more (PubMed: 25332161) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Glioma)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 25 µg
Specification Glioma
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 12 2015

Unfortunately, this antibody is not against the extracellular domain and so will not be suitable for your application.

We have not tested any of our antibodies in vivo and therefore cannot guarantee that any of these will work on live unpermea...

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Vielen Dank für Ihre Anfrage.

Ich habe Ihnen unser Subcellular Fractionation Protokoll an diese Email gehängt, mit dem Sie eine Isolierung der Zellmembran durchführen können.

Desweiteren habe ich Ihnen unseren &...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (gastric epithelial cell)
Specification gastric epithelial cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step DAKO serum free protein blocker as blocking agent for 20 minute(s) · Concentration: 0.25% · Temperature: 28°C
Username

Dr. J Chai

Verified customer

投稿 Apr 14 2012

Thank you for your inquiry.


Yes, the blocking buffer that was used to generate that image was a combination of 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protei...

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Thank you for contacting us. The blocking step requires you to choose either 1%BSA or 10% normal serum for blocking. Incubation should be either for one hour at room temperature or overnight at 4 degrees.


I hope this information is helpfu...

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Thank you for bringing this to our attention. Can you please send the order numbers for each antibody that has discrepancy between the delivered datasheet and the online datasheet? Please also send a brief description of the issues you are having, if a...

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Thank you for contacting us. We would guarantee this antibody to work in both human and mouse tissue sections.  If you had any difficulty in IHC-P with these two species, we would be able to replace or refund the products for you within 6 months of you...

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Thank you for contacting us. The antibody ab15459 has not yet been tested in flow cytometry. If you like to try that, we have a testing discount program you can take advantage of. For UNTESTED species and/or applications, we have estab...

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Thank you for contacting us. The C-terminal region is located in the cytoplasm. Depending on your studies, a permeabilization step would be necessary. I hope this information is helpful to you. Please do not hesitate to contact us if you need any m...

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"