Anti-Integrin beta 4 (phospho Y1526) 抗体 (ab29044)
Key features and details
- Rabbit polyclonal to Integrin beta 4 (phospho Y1526)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Integrin beta 4 (phospho Y1526) antibody
Integrin beta 4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Integrin beta 4 (phospho Y1526) -
由来種
Rabbit -
特異性
The immunogen sequence used is found in all three integrin beta4 isoforms and is highly conserved in rat and mouse integrin beta4. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Integrin beta 4 (phospho Y1526) conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- A431 and src-transformed Hct116 cells treated with pervanadate.
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特記事項
The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to dephosphorylated integrin beta4 (Tyr-1526) peptide before affinity purification using phospho-integrin beta-4(Tyr-1526) peptide (without carrier). -
一次抗体 備考
The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab29044の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000. Detects a band of approximately 202 kDa.
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特記事項 |
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WB
1/1000. Detects a band of approximately 202 kDa. |
ターゲット情報
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機能
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
組織特異性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
関連疾患
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
ドメイン
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3691 Human
- Entrez Gene: 192897 Mouse
- Entrez Gene: 25724 Rat
- Omim: 147557 Human
- SwissProt: P16144 Human
- SwissProt: A2A863 Mouse
- SwissProt: Q64632 Rat
- Unigene: 632226 Human
see all -
別名
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab29044 は 1 報の論文で使用されています。
- Pickl M & Ries CH Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene 28:461-8 (2009). WB ; Human . PubMed: 18978815