製品の概要

  • 製品名Anti-Integrin beta 4 antibody [ASC-8]
    Integrin beta 4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [ASC-8] to Integrin beta 4
  • アプリケーション適用あり: IP, IHC-Fr, Flow Cyt, Inhibition Assaymore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Human squamous cell carcinoma cells (SCC9)

  • ポジティブ・コントロール
    • IHC-Fr: Human epithelial tissues (including tongue, foreskin and ovary). Flow Cyt: Human SCC9, SKOV-3, HUVEC cell lines. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名ASC-8
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution. Precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
Inhibition Assay Use at an assay dependent dilution. Inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptide, but not to type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
    • 組織特異性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
    • 関連疾患Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • 配列類似性Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.
    • ドメインThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    Anti-Integrin beta 4 antibody [ASC-8] 画像

    • Overlay histogram showing A431 cells stained with ab77801 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77801, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Integrin beta 4 antibody [ASC-8] (ab77801) 使用論文

    This product has been referenced in:
    • Chen Q  et al. Down-regulation of Gli transcription factor leads to the inhibition of migration and invasion of ovarian cancer cells via integrin ß4-mediated FAK signaling. PLoS One 9:e88386 (2014). WB ; Human . Read more (PubMed: 24533083) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"