Anti-Integrin beta 4 抗体 [450-9D] (FITC) (ab22486)

製品の概要

  • 製品名
    Anti-Integrin beta 4 antibody [450-9D] (FITC)
    Integrin beta 4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [450-9D] to Integrin beta 4 (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    ab22486 recognises the integrin beta 4 expressed on epithelial cells, Schwann cells and various tumour cell lines.
  • アプリケーション
    適用あり: IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) (Human): Purified a6b4 integrin from A431 cells

  • エピトープ
    the epitope is in the extracellular region of Integrin beta 4
  • ポジティブ・コントロール
    • Human tonsil

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab22486 has been purified by affinity chromatography on Protein G from tissue culture supernatant.
  • ポリ/モノ
    モノクローナル
  • クローン名
    450-9D
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/10. Use 10ul of the suggested working dilution to label 106 cells in 100ul.



ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • 組織特異性
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • 関連疾患
    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • 配列類似性
    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • ドメイン
    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Anti-Integrin beta 4 antibody [450-9D] (FITC) 画像

  • Immunohistochemistry (Frozen sections) analysis of human tonsil tissue section labeling Integrin beta 4 with ab22486. 

Anti-Integrin beta 4 antibody [450-9D] (FITC) (ab22486) 使用論文

This product has been referenced in:
  • James JL  et al. Isolation and characterisation of a novel trophoblast side-population from first trimester placentae. Reproduction 150:449-62 (2015). Read more (PubMed: 26248480) »

See 1 Publication for this product

Product Wall

Further to previous correspondence I have been in touch with the source of this antibody. I am concerned that this antibody has in fact NOT been tested and shown to work by western blotting. It is FITC labeled and therefore my concern was that the fluo...

Read More

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I appreciate the controls that you have in place and that you are detecting Integrin beta 4 using an alternatively sourced antibody. Please ca...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録