Anti-Integrin beta 4 抗体 [346-11A] (ab25254)

製品の概要

  • 製品名
    Anti-Integrin beta 4 antibody [346-11A]
    Integrin beta 4 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [346-11A] to Integrin beta 4
  • 特異性
    ab25254 recognises Integrin ß4 chain (CD104), Mr 200 kDa.
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Full length native protein (purified) (Mouse) - Tumour associated antigen TSP 180 - immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    100mM borate buffered saline, pH 8.0.
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    346-11A
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 20094062
IHC-Fr Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 202 kDa.

ターゲット情報

  • 機能
    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • 組織特異性
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • 関連疾患
    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • 配列類似性
    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • ドメイン
    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

画像

  • This image shows a acetone fixed frozen section of BALB/c mouse intestine. The basal layer stains intensely (arrow).
  • Immunohistochemistry (Frozen sections) analysis of BALB/c mouse frozen intestine. Right: Integrin beta 4 labeled with ab25254 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Left: Isotype Control, Rat IgG2a-UNLB followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Blue - DAPI

  • ab25254 staining mouse small intestine tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 3% BSA for 1 hour at 37°C prior to incubatition with the primary antibody, diluted 1/500, for 12 hours at 4°C.  A Cy3® conjugated goat anti-rat antibody, diluted 1/200, was used as the secondary.

    See Abreview

  • ab25254 staining Intergrin beta 4 in mouse colon cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100) for 30 minutes at 20°C. An undiluted Alexa Fluor® 647-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Gating Strategy: Exclude small cells/debris.

    See Abreview

参考文献

This product has been referenced in:
  • Larkins CE  et al. Spatiotemporal dynamics of androgen signaling underlie sexual differentiation and congenital malformations of the urethra and vagina. Proc Natl Acad Sci U S A 113:E7510-E7517 (2016). Read more (PubMed: 27821748) »
  • Grudzien-Nogalska E  et al. CPEB1 promotes differentiation and suppresses EMT in mammary epithelial cells. J Cell Sci 127:2326-38 (2014). Read more (PubMed: 24634508) »

See all 6 Publications for this product

レビューと Q&A

Application
Flow Cytometry
Permeabilization
No
Sample
Mouse Cell (mouse colon)
Specification
mouse colon
Gating Strategy
exclude small cells/debris
Preparation
Cell harvesting/tissue preparation method: crypt isolation
Sample buffer: dmem
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投稿 Oct 03 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (colon epithelial)
Specification
colon epithelial
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 28 2014

DISCOUNT CODES:

ab25254 - ***
ab110167 - ***

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Small intestine)
Specification
Small intestine
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Apr 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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