Anti-Integrin beta 4 抗体 [346-11A] (ab25254)


  • 製品名Anti-Integrin beta 4 antibody [346-11A]
    Integrin beta 4 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [346-11A] to Integrin beta 4
  • 特異性ab25254 recognises Integrin ß4 chain (CD104), Mr 200 kDa.
  • アプリケーション適用あり: Flow Cyt, ICC/IF, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Full length native protein (purified) (Mouse) - Tumour associated antigen TSP 180 - immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー100mM borate buffered saline, pH 8.0.
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名346-11A
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野


Our Abpromise guarantee covers the use of ab25254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 20094062
IHC-Fr Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 202 kDa.


  • 機能Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • 組織特異性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • 関連疾患Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • 配列類似性Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • ドメインThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Anti-Integrin beta 4 antibody [346-11A] 画像

  • This image shows a acetone fixed frozen section of BALB/c mouse intestine. The basal layer stains intensely (arrow).
  • Immunohistochemistry (Frozen sections) analysis of BALB/c mouse frozen intestine. Right: Integrin beta 4 labeled with ab25254 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Left: Isotype Control, Rat IgG2a-UNLB followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Blue - DAPI

  • ab25254 staining mouse small intestine tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 3% BSA for 1 hour at 37°C prior to incubatition with the primary antibody, diluted 1/500, for 12 hours at 4°C.  A Cy3® conjugated goat anti-rat antibody, diluted 1/200, was used as the secondary.

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  • ab25254 staining Intergrin beta 4 in mouse colon cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100) for 30 minutes at 20°C. An undiluted Alexa Fluor® 647-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Gating Strategy: Exclude small cells/debris.

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Anti-Integrin beta 4 antibody [346-11A] (ab25254) 使用論文

This product has been referenced in:
  • Larkins CE  et al. Spatiotemporal dynamics of androgen signaling underlie sexual differentiation and congenital malformations of the urethra and vagina. Proc Natl Acad Sci U S A 113:E7510-E7517 (2016). Read more (PubMed: 27821748) »
  • Grudzien-Nogalska E  et al. CPEB1 promotes differentiation and suppresses EMT in mammary epithelial cells. J Cell Sci 127:2326-38 (2014). Read more (PubMed: 24634508) »

See all 6 Publications for this product

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Mouse Cell (mouse colon)
Specification mouse colon
Gating Strategy exclude small cells/debris
Preparation Cell harvesting/tissue preparation method: crypt isolation
Sample buffer: dmem

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Verified customer

投稿 Oct 03 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Cell (colon epithelial)
Specification colon epithelial
Permeabilization No
Fixative Formaldehyde

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Verified customer

投稿 Aug 28 2014


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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Small intestine)
Specification Small intestine
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

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Verified customer

投稿 Apr 03 2008