製品の概要

  • 製品名Anti-Integrin beta 1d antibody [2B1]
  • 製品の詳細
    Mouse monoclonal [2B1] to Integrin beta 1d
  • 特異性Recognizes specifically the cytoplasmic domain of integrin subunit beta 1D present in cardiac and skeletal muscle.
  • アプリケーション適用あり: WB, IHC-Fr, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    C-QENPIYKSPINNFKNPNYGRKAGL

    conjugated to KLH.

  • エピトープCytoplasmic domain, C-terminal. Isoform of the Integrin b1 subunit present in cardiac and skeletal muscle.
  • 特記事項


    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.
  • ポリ/モノモノクローナル
  • クローン名2B1
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/1000.
IHC-Fr 1/25 - 1/200.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 関連性Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. The isoform beta-1D of Integrin 1, is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • 細胞内局在Cell Membrane
  • 参照データベース
  • 別名
    • CD29 antibody
    • FNRB antibody
    • Integrin beta 1 antibody
    • ITGB 1 antibody
    • ITGB1 antibody
    • MDF2 antibody
    • MSK12 antibody
    • VLAB antibody
    see all

Anti-Integrin beta 1d antibody [2B1] 画像

  • Western blotting of beta1D integrin showing its muscle cell specific expression pattern.
  • Immunofluorescence staining of myocardium for beta1D integrin.

Anti-Integrin beta 1d antibody [2B1] (ab8991) 使用論文

This product has been referenced in:
  • Barton ER  et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J : (2012). ICC/IF ; Mouse . Read more (PubMed: 22649033) »
  • Barton ER Restoration of gamma-sarcoglycan localization and mechanical signal transduction are independent in murine skeletal muscle. J Biol Chem 285:17263-70 (2010). WB ; Mouse . Read more (PubMed: 20371873) »

See all 3 Publications for this product

Product Wall

Please forward the following publication to customer, there are many markers listed and we have antibodies against these. Customer might need to make decision based on their requirements.

http://journals.tums.ac.ir/upload_files/pdf/_/20047.pdf...

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I would recommend to use an anti-integrin beta 1d such as http://www.abcam.com/ab8992 or http://www.abcam.com/ab8991.

Thank you for your enquiry. I am sorry it has taken a little time to obtain this protocol for you. Thank you for your patience. I hope this will be helpflu to you. Please note that this procedure would be a guideline only and may require some fur...

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We have just received the following information from the source of ab8991: "We would suggest to increase the protein concentration in the gel and to use more concentrated antibody for the blotting as this antibody is supplied as tissue culture supe...

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Thank you for your enquiry. For Western blotting, I would suggest starting at a dilution of 1:1000. For IHC, I would recommend titrating the antibody 1:20 to 1:200. For both applications, you will need to optimize based upon results. Please contact...

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We would expect this antibody to work on rat and mouse tissues, but cannot guarantee it. This epitope is conserved across species.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"