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Tissue/ cell preparation (Human): Burkitt's lymphoma cell line Raji.
The antibody was conjugated with Biotin-LC-NHS. It is free of unconjugated biotin.
Our Abpromise guarantee covers the use of ab28100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/1000. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
5 µm streptavidin coated silica microspheres (Bangs Laboratories) were washed. ab28100 (1/500) diluted in BSA/PBS was added to the spheres and left at room temperature under rotation to bind. The coated spheres were added to HUVEC cells. They were caught individually in an optical trap and brought into contact to the cells to attach (a). Afterwards the sphere was pulled away from the cells and a tether formed that could elongate until a surplus of a membrane existed. At that point the sphere escaped from the optical trap and together with the tether it retracted back to the cell (b).
ab28100 has not yet been referenced specifically in any publications.