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Human umbilical vein EC.
Our Abpromise guarantee covers the use of ab7168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. See Abreview.|
|Flow Cyt||Use 1µg for 106 cells. (paraformaldehyde or methanol fixed cells).
|Inhibition Assay||Use at an assay dependent concentration. The antibody has been functionally tested by adhesion assay, chemotaxis, patch clamp analysis and neurotoxicity induced apoptosis.|
Overlay histogram showing MCF-7 cells stained with ab7168 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7168, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.