Anti-Integrin alpha V+beta 5 抗体 [P1F6] (ab24694)

製品の概要

  • 製品名Anti-Integrin alpha V+beta 5 antibody [P1F6]
    Integrin alpha V+beta 5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [P1F6] to Integrin alpha V+beta 5
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IPmore details
  • 種交差性
    交差種: Rat, Hamster, Human
  • 免疫原

    Tissue/ cell preparation (Human) - small cell lung carcinoma cells (UCLA P3).

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 3rd October 2016. The following lots are from ascites and are still in stock as of 3rd October 2016 (GR290948, GR243624 and GR207301). Lot numbers higher than GR290948 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Prepared using ascites purified sample. Use at an assay dependent concentration.

ターゲット情報

  • 関連性This protein belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
  • 細胞内局在Cell Membrane
  • 参照データベース
  • 別名
    • CD51 antibody
    • Integrin alpha V antibody
    • Integrin beta 5 antibody
    • ITGAV antibody
    • ITGB5 antibody
    • VNRA antibody
    see all

Anti-Integrin alpha V+beta 5 antibody [P1F6] (ab24694) 使用論文

This product has been referenced in:
  • Lee P  et al. A novel cell adhesion region in tropoelastin mediates attachment to integrin aVß5. J Biol Chem 289:1467-77 (2014). Human . Read more (PubMed: 24293364) »
  • Buchholz DE  et al. Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium. Stem Cells Transl Med 2:384-93 (2013). Human . Read more (PubMed: 23599499) »

See all 6 Publications for this product

Product Wall

Application ChIP on chip
Sample Rat Cell lysate - other (neural)
Negative control Functionalization test of gold surfaces with SAM with non-activated carboxythiols plus FITC conjugated secondary antibody.
Specification neural
Detection step Microarray
Positive control Functionalization test of gold surfaces with SAM with activated carboxythiols and ab24694 antibody. revealed with FITC conjugated secondary antibody
Username

Abcam user community

Verified customer

投稿 Jun 15 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Primary mouse RPE)
Specification Primary mouse RPE
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Fixative Paraformaldehyde
Username

Gareth Ward

Verified customer

投稿 May 28 2015

Thank you for contacting us.

This antibody is effective in immunoprecipitation (IP) of the αvβ5 complex under non-reducing conditions from surface-labelled cell lysates and can also be detected in focal adhesions by immunofluoresce...

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Thank you for contacting us.

Please note that if a product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Su...

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Thank you for contacting Abcam.

The antibody ab24694 does recognize both the alpha V+beta 5 subunits of Intergrin and based off the Uniprot sequences for both these subunits, thebands that you are seeing could very well these subunits as both...

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Thank you for contacting Abcam. I have checked our notes and it appears that ab7166 is unsuitable for western blot, while ab24694 will most probably work for western blotting, its just that we have not tested it in that application. Please let me know ...

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Thank you for contacting Abcam regarding ab24694.   We have tested it on paraffin embedded sections with good results. We have not tested it for use in flow cytometry and cannot guarantee that it will work. We also have not done any epitope mapping...

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Thank you for contacting us. The immunogen for ab24694 has not been mapped. The 3 antibodies (ab24694, ab78454 and ab79340) we have for Integrin alpha V+beta 5 have not yet tested in mouse samples. I have checked the sequence homology be...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Breast Cancer Cells)
Specification Breast Cancer Cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Sharon Sneddon

Verified customer

投稿 Oct 22 2008

Thank you for your enquiry. I can confirm that this antibody (ab24694) will detect both integrin alpha 5 and integrin beta 5 under non reducing conditions in western blotting. There is a reference on the datasheet which the antibody has been us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"