Anti-Integrin alpha V + beta 6 抗体 [10D5] (ab77906)

製品の概要

  • 製品名
    Anti-Integrin alpha V + beta 6 antibody [10D5]
    Integrin alpha V + beta 6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10D5] to Integrin alpha V + beta 6
  • 特異性
    ab77906 recognises alpha V beta 6 and does not recognize alpha V or other alpha V integrins.
  • アプリケーション
    適用あり: IP, Flow Cyt, Blockingmore details
    適用なし: IHC-P or WB
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Immunization of beta 6 knockout mice with wild type murine keratinocytes.

  • ポジティブ・コントロール
    • H441 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture.
Flow Cyt Use at an assay dependent concentration. Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Blocking Use a concentration of 100 µg/ml. Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP.
  • 追加情報
    Is unsuitable for IHC-P or WB.
  • ターゲット情報

    参考文献

    This product has been referenced in:
    • de la Mare JA  et al. Extracellular Hsp90 and TGFß regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model. BMC Cancer 17:202 (2017). Human . Read more (PubMed: 28302086) »
    • Bian Q  et al. Mechanosignaling activation of TGFß maintains intervertebral disc homeostasis. Bone Res 5:17008 (2017). Mouse . Read more (PubMed: 28392965) »

    See all 4 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Dec 29 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP - prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    LNCaP - prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Username

    Dr. Armen Petrosyan

    Verified customer

    投稿 Dec 29 2017

    The exact target epitope was not characterized in-house. Please see the references listed above for more details.
    We do not have information on this product's reactivity with mouse as this antibody has, to our knowledge, not been tested on mouse sa...

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    Thank you for contacting us.

    This antibody (ab78438) recognizes the alpha V beta 5 heterodimer, not alpha V beta 6. We do however offer another product which does recognize alpha V beta 6. That product number is ab77906 and may be found at th...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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