Anti-Integrin alpha V beta 3 抗体 [27.1 (VNR-1)] (ab78289)

製品の概要

  • 製品名
    Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)]
    Integrin alpha V beta 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [27.1 (VNR-1)] to Integrin alpha V beta 3
  • 特異性
    Reacts with the Integrin alpha V beta 3
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, IP, IHC-Fr, Blockingmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 22655084
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. 4-10% formalin fixed frozen tissues (short fixation times, 10 minutes room temperature) only'
Blocking Use at an assay dependent concentration. Competes with clone LM609 for integrin function blocking.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 関連性
      Function: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C (PubMed:20615244). Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for coxsackievirus A9 and B1 (PubMed:9426447, PubMed:15194773, PubMed:7519807). Integrin ITGAV:ITGB3 acts as a receptor for herpes virus 8/HHV-8 (PubMed:18045938). Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1 (PubMed:24367260). Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1 (PubMed:11160695). Integrin ITGAV:ITGB3 acts as a receptor for West nile virus (PubMed:23658209). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
    • 細胞内局在
      Cell Membrane. Cytoplasmic. Integrin complex. Focal adhesion.
    • 参照データベース
    • 別名
      • Antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD 61 antibody
      • CD51 antibody
      • CD51 antigen antibody
      • CD61 antibody
      • CD61 antigen antibody
      • DKFZp686A08142 antibody
      • GP3A antibody
      • GPIIIa antibody
      • Integrin alpha V antibody
      • Integrin alpha V vitronectin receptor alpha polypeptide antibody
      • Integrin alpha V vitronectin receptor alpha polypeptide antigen CD51 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • Integrin beta 3 antibody
      • Integrin beta 3 platelet glycoprotein IIIa antigen CD61 antibody
      • Integrin beta chain beta 3 antibody
      • integrinVbeta3 antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • MSK 8 antibody
      • MSK8 antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • Vitronectin receptor alpha polypeptide antibody
      • vitronectin receptor antibody
      • Vitronectin receptor subunit alpha antibody
      • VNR 1 antibody
      • VNR1 antibody
      • VNRA antibody
      see all

    画像

    • Immunofluorescence analysis of U87MG cells, staining Integrin alpha V beta 3 (red) with ab78289 at 1/500 dilution.

      Cells were either untreated (left) or treated with 10 nM PTX for 12 hours (right). Nuclei were stained with DAPI (blue).
    • Overlay histogram showing A431 cells stained with ab78289 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78289, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献

    This product has been referenced in:

    See all 16 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Candida albicans Cell (Hypha Cell)
    Permeabilization
    Yes - Saponin
    Specification
    Hypha Cell
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Angela Lopez

    Verified customer

    投稿 Jun 15 2016

    Thank you for contacting us.

    The hybridoma was generated from B cell isolated from a mouse immunized with alphaVbeta3, the monoclonal produced from the hybridoma recoginzes a conformational epitope. We do not know whether this monoclonal recog...

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    Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the ...

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    Thank you for contacting us. I received the following information from the lab: The antibody was tested for use in IHC by external academic laboratories. The researchers used human heart artery segments, that were snap frozen in OCT. ...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Embryonic Fibroblast (MEF))
    Specification
    Mouse Embryonic Fibroblast (MEF)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton
    Username

    Abcam user community

    Verified customer

    投稿 Sep 16 2011

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cell line ( cervical cancer))
    Specification
    HeLa cell line ( cervical cancer)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton
    Username

    Abcam user community

    Verified customer

    投稿 Sep 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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