製品の概要

  • 製品名Anti-Integrin alpha V antibody [P2W7]
    Integrin alpha V 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [P2W7] to Integrin alpha V
  • アプリケーション適用あり: IP, ICC/IF, WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    An ocular melanoma cell line expressing high levels of human integrin alpha V beta 1.

  • ポジティブ・コントロール
    • Tested with squamous cell carcinoma cells. A375M melanoma cell line. A549 cell line IF/ICC
  • 特記事項Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 - 10 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD51 antibody
    • DKFZp686A08142 antibody
    • integrin alpha V beta 3 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • integrin alphaVbeta3 antibody
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • MSK 8 antibody
    • Msk8 antibody
    • Vitronectin receptor subunit alpha antibody
    • VNRA antibody
    • VTNR antibody
    see all

Anti-Integrin alpha V antibody [P2W7] 画像

  • ICC/IF image of ab11470 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11470, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, a goat anti-mouse DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing A549 cells stained with ab11470 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11470, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed A549 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Integrin alpha V antibody [P2W7] (ab11470) 使用論文

This product has been referenced in:
  • Farsinejad A  et al. Classification of Iranian patients with Glanzmann's Thrombasthenia using a flow cytometric method. Platelets 22:321-7 (2011). Read more (PubMed: 21526886) »
  • Wang C  et al. transient scrotal hyperthermia and levonorgestrel enhance testosterone-induced spermatogenesis suppression in men through increased germ cell apoptosis. J Clin Endocrinol Metab 92:3292-304 (2007). Read more (PubMed: 17504903) »

See all 3 Publications for this product

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