Anti-Integrin alpha V 抗体 [272-17E6] (ab16821)

製品の概要

  • 製品名
    Anti-Integrin alpha V antibody [272-17E6]
    Integrin alpha V 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [272-17E6] to Integrin alpha V
  • 特異性
    Cross reacts with all alpha V integrins.
  • アプリケーション
    適用あり: Inhibition Assay, ICC/IF, Blocking, Flow Cyt, ELISA, IPmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Full length native protein (purified) (Human).

  • ポジティブ・コントロール
    • Human breast carcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Inhibition Assay Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 19115199
Flow Cyt Use a concentration of 2.5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
IP Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能
    The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD51 antibody
    • DKFZp686A08142 antibody
    • integrin alpha V beta 3 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • integrin alphaVbeta3 antibody
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • MSK 8 antibody
    • Msk8 antibody
    • Vitronectin receptor subunit alpha antibody
    • VNRA antibody
    • VTNR antibody
    see all

Anti-Integrin alpha V antibody [272-17E6] 画像

  • ab16821 staining Integrin alpha V in Human prostate cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/250 in 1% BSA/PBS) for 15 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as secondary antibody.

    See Abreview

  • ab16821 staining Integrin alpha V in Human melanoma cells by Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0.1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

    See Abreview

Anti-Integrin alpha V antibody [272-17E6] (ab16821) 使用論文

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). ICC/IF ; Human . Read more (PubMed: 26732624) »
  • Baldwin AK  et al. Epithelial-mesenchymal status influences how cells deposit fibrillin microfibrils. J Cell Sci 127:158-71 (2014). Blocking ; Human . Read more (PubMed: 24190885) »

See all 11 Publications for this product

Product Wall

Vielen Dank für Ihre Anfrage.

Das Labor, von welchem wir diesen Antikörper beziehen, hat uns tatsächlich informiert, dass dieser Antikörper nicht auf Paraffinschnitten funktionieren sollte. Dies ist eine Information von de...

Read More

The exact target epitope was not characterized in-house. Please see the references listed above for more details.
We do not have information on this product's reactivity with mouse as this antibody has, to our knowledge, not been tested on mouse sa...

Read More

I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this ba...

Read More

I am sorry about the issues you have been having. Thank you for bringing this to our attention.

I would very much like to help you with this. However to do that I need the particulars of your protocol. This information is important not only ...

Read More

Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Username

Abcam user community

Verified customer

投稿 Aug 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell line ( cervical cancer))
Specification
HeLa cell line ( cervical cancer)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Username

Abcam user community

Verified customer

投稿 Aug 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (melanoma cell line)
Specification
melanoma cell line
Preparation
Cell harvesting/tissue preparation method: cell line - trypsinized cells, treated with STI and spun out trypsin, kept on ice during experiment duration
Sample buffer: 2% FCS/0.1%NaAzide/PBS
Fixation
none
Permeabilization
No
Gating Strategy
FSC and SSC for healthy cell population, excluded PI positive cells
Username

Abcam user community

Verified customer

投稿 Oct 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Specification
prostate
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録