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Full length native protein (purified) (Human).
Our Abpromise guarantee covers the use of ab16821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Inhibition Assay||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration. PubMed: 19115199|
|Flow Cyt||Use a concentration of 2.5 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use a concentration of 2.5 µg/ml.|
ab16821 staining Integrin alpha V in Human prostate cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/250 in 1% BSA/PBS) for 15 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as secondary antibody.
ab16821 staining Integrin alpha V in Human melanoma cells by Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0.1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.