Anti-Integrin alpha 9+beta 1 抗体 [Y9A2] (ab27947)

製品の概要

  • 製品名
    Anti-Integrin alpha 9+beta 1 antibody [Y9A2]
  • 製品の詳細
    Mouse monoclonal [Y9A2] to Integrin alpha 9+beta 1
  • 特異性
    Ab27947 recognises alpha9 integrin expressed as a heterodimer in association with the beta1 integrin.
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, IP, WB, ICCmore details
    適用なし: IHC-FoFr,IHC-P or IHC-R
  • 種交差性
    交差種: Rat, Guinea pig, Human
  • 免疫原

    Tissue/ cell preparation (Human): alpha9 integrin transfected lambda cells.

  • 特記事項


    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Ab27947 was purified by affinity chromatography, from tissue culture supernatant.
  • 一次抗体 備考
    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.
  • ポリ/モノ
    モノクローナル
  • クローン名
    Y9A2
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20810886
Flow Cyt Use 10µl for 106 cells.

(in 100µl). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 20810886
ICC Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IHC-FoFr,IHC-P or IHC-R.
  • ターゲット情報

    • 関連性
      Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Integrin alpha 9, when bound to the integrin beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of integrin alpha 9 has been found to be upregulated in small cell lung cancers.
    • 細胞内局在
      Type I membrane protein.
    • 参照データベース
    • 別名
      • CD29 antibody
      • FNRB antibody
      • GPIIA antibody
      • ITGA9 antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • VLAB antibody
      see all

    Anti-Integrin alpha 9+beta 1 antibody [Y9A2] (ab27947) 使用論文

    This product has been referenced in:
    • Franssen EH  et al. Exclusion of integrins from CNS axons is regulated by Arf6 activation and the AIS. J Neurosci 35:8359-75 (2015). Read more (PubMed: 26019348) »
    • Ou J  et al. Fibronectin extra domain A (EDA) sustains CD133(+)/CD44(+) subpopulation of colorectal cancer cells. Stem Cell Res 11:820-33 (2013). Read more (PubMed: 23811539) »

    See all 6 Publications for this product

    Product Wall

    Thank you for your enquiry.

    The following information is taken from Human Protein Atlas.

    1) Integrin alpha 9 positive control: Digestive tract
    http://www.proteinatlas.org/ENSG00000144668

    2) Integrin beta 1 positive...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Endothelial cells and fibroblasts cultured from hu)
    Specification
    Endothelial cells and fibroblasts cultured from hu
    Fixative
    Methanol
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Sep 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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