Anti-Integrin alpha 9+beta 1 抗体 [Y9A2] (ab27947)


  • 製品名Anti-Integrin alpha 9+beta 1 antibody [Y9A2]
  • 製品の詳細
    Mouse monoclonal [Y9A2] to Integrin alpha 9+beta 1
  • 特異性Ab27947 recognises alpha9 integrin expressed as a heterodimer in association with the beta1 integrin.
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IP, WB, ICCmore details
    適用なし: IHC-FoFr,IHC-P or IHC-R
  • 種交差性
    交差種: Rat, Guinea pig, Human
  • 免疫原

    Tissue/ cell preparation (Human): alpha9 integrin transfected lambda cells.

  • 特記事項

    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Ab27947 was purified by affinity chromatography, from tissue culture supernatant.
  • 一次抗体 備考This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.
  • ポリ/モノモノクローナル
  • クローン名Y9A2
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab27947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20810886
Flow Cyt Use 10µl for 106 cells.

(in 100µl). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 20810886
ICC Use at an assay dependent concentration.
  • 追加情報Is unsuitable for IHC-FoFr,IHC-P or IHC-R.
  • ターゲット情報

    • 関連性Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Integrin alpha 9, when bound to the integrin beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of integrin alpha 9 has been found to be upregulated in small cell lung cancers.
    • 細胞内局在Type I membrane protein.
    • 参照データベース
    • 別名
      • CD29 antibody
      • FNRB antibody
      • GPIIA antibody
      • ITGA9 antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • VLAB antibody
      see all

    Anti-Integrin alpha 9+beta 1 antibody [Y9A2] (ab27947) 使用論文

    This product has been referenced in:
    • Ou J  et al. Fibronectin extra domain A (EDA) sustains CD133(+)/CD44(+) subpopulation of colorectal cancer cells. Stem Cell Res 11:820-33 (2013). Read more (PubMed: 23811539) »
    • Eva R  et al. Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells. J Neurosci 30:11654-69 (2010). ICC/IF, Inhibition Assay ; Rat . Read more (PubMed: 20810886) »

    See all 5 Publications for this product

    Product Wall

    Thank you for your enquiry.

    The following information is taken from Human Protein Atlas.

    1) Integrin alpha 9 positive control: Digestive tract

    2) Integrin beta 1 positive...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cultured Cells (Endothelial cells and fibroblasts cultured from hu)
    Specification Endothelial cells and fibroblasts cultured from hu
    Fixative Methanol
    Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C

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    投稿 Sep 16 2011