Anti-Integrin alpha 6 抗体 [GoH3] (Phycoerythrin) (ab95703)

製品の概要

  • 製品名Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin)
    Integrin alpha 6 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [GoH3] to Integrin alpha 6 (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション適用あり: Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Cow, Human
  • 免疫原

    Mouse mammary tumor cells

  • ポジティブ・コントロール
    • Normal Human peripheral blood cells/leukocytes
  • 特記事項


    The GoH3 antibody has been reported to have functional activity in blocking the binding of Integrin alpha 6 to laminin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.



ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 23613946

ターゲット情報

  • 機能Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • 組織特異性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • 関連疾患Epidermolysis bullosa letalis, with pyloric atresia
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 翻訳後修飾Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • 細胞内局在Cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) 画像

  • Immunocytochemistry/ Immunofluorescence analysis of non-GFP positive beta-TC6 cells on laminin-coated cover slips labeling Integrin alpha 6 with ab95703 at 1/100 dilution.
    Cells were fixed in 4% buffered paraformaldehyde at room temperature for 5 minutes then washed with PBS prior to permeabilisation and blocking using PBS with 0.1% triton® X-100, 1% BSA, and 3% fetal calf serum. The cells were incubated with Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703) in PBS with 1% BSA and 1% fetal calf serum for 30 minutes at 37°C before washing two times with PBS. The cultures were then incubated with secondary antibody for 30 minutes at 37°C and rinsed three times in PBS for 15 minutes, the second wash included Hoechst (11 ng/mL). Coverslips were mounted on glass slides with fluorescent mounting solution. Fluorescence was photographed in a fluorescence microscope using a 20× lens.

  • Flow Cytometry analysis of normal human peripheral blood cells stained with Rat IgG2a kappa Isotype Control Phycoerythrin (blue histogram) or Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703) (purple histogram). Cells in the lymphocyte gate were used for analysis.

  • Immunocytochemistry/ Immunofluorescence analysis of Non-GFP positive beta-TC6 cells cultured with GFP-positive NCSC cells (green cells) on laminin-coated cover slips labeling Integrin alpha 6 with ab95703 at 1/100 dilution (orange).
    Cells were fixed in 4% buffered paraformaldehyde at room temperature for 5 minutes then washed with PBS prior to permeabilisation and blocking using PBS with 0.1% triton® X-100, 1% BSA, and 3% fetal calf serum. The cells were incubated with Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703) in PBS with 1% BSA and 1% fetal calf serum for 30 minutes at 37°C before washing two times with PBS. The cultures were then incubated with secondary antibody for 30 minutes at 37°C and rinsed three times in PBS for 15 minutes, the second wash included Hoechst (11 ng/mL). Coverslips were mounted on glass slides with fluorescent mounting solution. Fluorescence was photographed in a fluorescence microscope using a 20× lens.

  • Flow cytometry staining of normal Human peripheral blood cells with 0.06 µg of Rat IgG2a Isotype Control PE (open histogram) or 0.06 µg of ab95703 (filled histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.

Anti-Integrin alpha 6 antibody [GoH3] (Phycoerythrin) (ab95703) 使用論文

This product has been referenced in:
  • Ngamjariyawat A  et al. Co-culture of neural crest stem cells (NCSC) and insulin producing beta-TC6 cells results in cadherin junctions and protection against cytokine-induced beta-cell death. PLoS One 8:e61828 (2013). ICC/IF ; Mouse . Read more (PubMed: 23613946) »
  • Ambrose HE & Wagner SD Alpha6-integrin is expressed on germinal centre B cells and modifies growth of a B-cell line. Immunology 111:400-6 (2004). Flow Cyt . Read more (PubMed: 15056376) »

See all 3 Publications for this product

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For ab95703 (Anti-Human/Mouse CD49f (Integrin alpha 6) PE), it is a cell surface staining antibody. We test this antibody in-house as a cell surface stain on normal h...

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