製品の概要

  • 製品名
    Anti-Integrin alpha 6 antibody [GoH3] (Biotin)
    Integrin alpha 6 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [GoH3] to Integrin alpha 6 (Biotin)
  • 標識
    Biotin
  • 特異性
    ab28080 recognises CD49F (VLA-6 alpha chain)
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse, Dog, Human, Cynomolgus monkey
  • 免疫原

    Tissue/ cell preparation (Mouse) BALB/c mouse mammary tumour cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報
    Flow Cyt: Use a working dilution of neat to 1/5. Use 10µl for 106 cells in 100ul.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
    • 組織特異性
      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
    • 関連疾患
      Epidermolysis bullosa letalis, with pyloric atresia
    • 配列類似性
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • 翻訳後修飾
      Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
      In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
      Palmitoylation by DHHC3 enhances stability and cell surface expression.
    • 細胞内局在
      Cell membrane. Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha6p antibody
      • CD49 antigen-like family member F antibody
      • CD49f antibody
      • Integrin alpha6B antibody
      • Integrin, alpha 6 antibody
      • ITA6_HUMAN antibody
      • ITGA6 antibody
      • ITGA6B antibody
      • Processed integrin alpha-6 antibody
      • VLA 6 antibody
      • VLA-6 antibody
      see all

    画像

    • ab28080, at 10µl per 106 cells, staining human Integrin alpha 6 in peripheral blood lymphocytes by Flow Cytometry

    プロトコール

    参考文献

    This product has been referenced in:
    • Lim E  et al. Aberrant luminal progenitors as the candidate target population for basal tumor development in BRCA1 mutation carriers. Nat Med 15:907-13 (2009). Read more (PubMed: 19648928) »
    • Yoshino N  et al. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim 49:97-110 (2000). Read more (PubMed: 10889948) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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