製品の概要

  • 製品名
    Anti-Integrin alpha 6 antibody [GoH3]
    Integrin alpha 6 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [GoH3] to Integrin alpha 6
  • アプリケーション
    適用あり: IP, ICC/IF, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab105669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ICC/IF 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 19762710
Flow Cyt Use at an assay dependent concentration.

This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • 組織特異性
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • 関連疾患
    Epidermolysis bullosa letalis, with pyloric atresia
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 翻訳後修飾
    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • 細胞内局在
    Cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Anti-Integrin alpha 6 antibody [GoH3] 画像

  • ab105669 staining Integrin alpha 6 in undifferentiated human embryonic stem cells on a murine embryonic fibroblast (MEFs) feeding layer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized and blocked with 1% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in 1% serum, 0.1% triton, 0.1% BSA + PBS) for 16 hours at 4°C. An Alexa Flour® 488-conjugated donkey anti-rat IgG polyclonal was used as the secondary antibody at a dilution of 1/500.

  • Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (open histogram)or 0.25 ug of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE Cells in the lymphocyte gate were used for analysis

Anti-Integrin alpha 6 antibody [GoH3] (ab105669) 使用論文

This product has been referenced in:

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 Feb 23 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
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投稿 Nov 18 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Nov 13 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
0.1% BSA, 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (Embryonic stem cell)
Specification
Embryonic stem cell
Permeabilization
Yes - 0.1% triton X
Fixative
methanol:acetone
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投稿 Apr 04 2014

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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投稿 Jan 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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