製品の概要

  • 製品名Anti-Integrin alpha 6 antibody
    Integrin alpha 6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Integrin alpha 6
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human Integrin alpha 6.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Small Intestine tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • 組織特異性Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • 関連疾患Epidermolysis bullosa letalis, with pyloric atresia
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 翻訳後修飾Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • 細胞内局在Cell membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Anti-Integrin alpha 6 antibody 画像

  • Anti-Integrin alpha 6 antibody (ab112181) at 1 µg/ml + Human small intestine tissue lysate - total protein (ab29276) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 126 kDa
    Observed band size : 126 kDa
    Additional bands at : 82 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • IHC image of Integrin alpha 6 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112181, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Integrin alpha 6 antibody (ab112181) 使用論文

ab112181 has not yet been referenced specifically in any publications.

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