製品の概要
- 製品名Anti-Integrin alpha 5 antibody
Integrin alpha 5 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to Integrin alpha 5
- アプリケーション適用あり: WB, IHC-Pmore details
- 種交差性交差種: Human
交差が予測される動物種: Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human Integrin alpha 5.
- ポジティブ･コントロール
- WB: This antibody gave a positive signal in Human Placenta tissue lysate, JEG-3 whole cell lysate, HeLa whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - 精製度Immunogen affinity purified
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab112183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 114 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. |
ターゲット情報
- 機能Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
- 配列類似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. - 細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 3678 Human
- Omim: 135620 Human
- SwissProt: P08648 Human
- Unigene: 505654 Human
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別名
- CD49 antigen-like family member E antibody
- CD49e antibody
- Fibronectin receptor subunit alpha antibody
see all
Anti-Integrin alpha 5 antibody 画像
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Western blot - Anti-Integrin alpha 5 antibody (ab112183)
Predicted band size : 114 kDaLane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Integrin alpha 5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jeg3 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab112183 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab112183 was shown to recognize Integrin alpha 5 when Integrin alpha 5 knockout samples were used, along with additional cross-reactive bands. Wild-type and Integrin alpha 5 knockout samples were subjected to SDS-PAGE. Ab112183 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 5 antibody (ab112183)IHC image of Integrin alpha 5 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112183, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Integrin alpha 5 antibody (ab112183)All lanes : Anti-Integrin alpha 5 antibody (ab112183) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 114 kDa
Observed band size : 145 kDa (why is the actual band size different from the predicted?)
Additional bands at : 32 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 secondsIntegrin alpha 5 contains an extensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Anti-Integrin alpha 5 antibody (ab112183) 使用論文
ab112183 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"