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Tissue/ cell preparation of C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)
The monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.
Our Abpromise guarantee covers the use of ab25189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|Blocking||Use at an assay dependent concentration.
In vitro blocking of adhesion.
|IHC-Fr||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.
ab25189 has not yet been referenced specifically in any publications.