Anti-Integrin alpha 4 抗体 [TA-2] (ab119337)

製品の概要

  • 製品名Anti-Integrin alpha 4 antibody [TA-2]
    Integrin alpha 4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TA-2] to Integrin alpha 4
  • アプリケーション適用あり: IP, ELISA, Flow Cyt, IHC-P, Neutralising, Blockingmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Rat Integrin alpha 4

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. 5ul per million cells



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 1 - 10 µg/ml.
Neutralising Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.

ターゲット情報

  • 機能Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • ドメインThe SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
  • 翻訳後修飾Phosphorylation on Ser-1027 inhibits PXN binding.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 269C wild type antibody
    • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
    • CD49 antigen like family member D antibody
    • CD49 antigen-like family member D antibody
    • CD49d antibody
    • IA4 antibody
    • Integrin alpha 4 antibody
    • Integrin alpha 4 subunit antibody
    • Integrin alpha IV antibody
    • Integrin alpha-4 antibody
    • Integrin alpha-IV antibody
    • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
    • ITA4_HUMAN antibody
    • ITGA4 antibody
    • MGC90518 antibody
    • OTTHUMP00000205320 antibody
    • Very late activation protein 4 receptor, alpha 4 subunit antibody
    • VLA 4 subunit alpha antibody
    • VLA-4 subunit alpha antibody
    • VLA4 antibody
    see all

Anti-Integrin alpha 4 antibody [TA-2] 画像

  • Flow cytometry analysis of Integrin alpha 4 in THP-1 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Integrin alpha 4 monoclonal antibody (ab119337) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow cytometry analysis of Integrin alpha 4 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Integrin alpha 4 monoclonal antibody (ab119377) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow cytometry analysis of Integrin alpha 4 in C6 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Integrin alpha 4 monoclonal antibody (ab119337) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Anti-Integrin alpha 4 antibody [TA-2] (ab119337) 使用論文

ab119337 has not yet been referenced specifically in any publications.

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