製品の概要

  • 製品名
    Anti-Integrin alpha 4 antibody [PS/2]
    Integrin alpha 4 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [PS/2] to Integrin alpha 4
  • アプリケーション
    適用あり: Inhibition Assay, Flow Cyt, IP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項

    This antibody has also been shown to be useful in: Studies of cell to cell interaction. Studies of tumour cell metastasis and adhesion. Inhibition of lymphopoiesis in Whitlock Witte long term bone marrow cultures. Retardation of myelopoiesis in Dexter type cultures.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Inhibition Assay Use at an assay dependent concentration. PubMed: 1997648
Flow Cyt Use at an assay dependent concentration. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • ドメイン
    The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
  • 翻訳後修飾
    Phosphorylation on Ser-1027 inhibits PXN binding.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 269C wild type antibody
    • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
    • CD49 antigen like family member D antibody
    • CD49 antigen-like family member D antibody
    • CD49d antibody
    • IA4 antibody
    • Integrin alpha 4 antibody
    • Integrin alpha 4 subunit antibody
    • Integrin alpha IV antibody
    • Integrin alpha-4 antibody
    • Integrin alpha-IV antibody
    • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
    • ITA4_HUMAN antibody
    • ITGA4 antibody
    • MGC90518 antibody
    • OTTHUMP00000205320 antibody
    • Very late activation protein 4 receptor, alpha 4 subunit antibody
    • VLA 4 subunit alpha antibody
    • VLA-4 subunit alpha antibody
    • VLA4 antibody
    see all

画像

  • Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling Integrin alpha 4 with ab25247 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-AF647 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-AF647.

  • Overlay histogram showing Human B Cells stained with ab25427 (blue line). Cells were kept in PBS buffer. ab25247 was diluted 1/50 and incubated with the product for 15 minutes at 4°C. The secondary antibody was Allophyocyanin-conjugated goat anti-rat polyclonal, diluted 1/50. Isotype control antibody (red line) was used under the same conditions. Cell population was gated on live cells.

    See Abreview

参考文献

This product has been referenced in:
  • Totsuka N  et al. Toll-like receptor 4 and MAIR-II/CLM-4/LMIR2 immunoreceptor regulate VLA-4-mediated inflammatory monocyte migration. Nat Commun 5:4710 (2014). Flow Cyt ; Mouse . Read more (PubMed: 25134989) »
  • Castro FV  et al. 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype. Leukemia 26:1487-98 (2012). Flow Cyt . Read more (PubMed: 22266911) »

See all 4 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (B CELLS)
Specification
B CELLS
Permeabilization
No
Gating Strategy
gated on live cells
Username

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Verified customer

投稿 May 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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