製品の概要

  • 製品名Anti-Integrin alpha 3 antibody [J143]
    Integrin alpha 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [J143] to Integrin alpha 3
  • 特異性This antibody recognizes the alpha 3 subunit of VLA3 (ECMRI - extracellular matrix receptor I, URO1), which has been shown to be related to the chicken fibronectin/laminin receptor. VLA proteins are part of the integrin family of cell adhesion molecules, and the distribution of VLA3 in cells and tissues suggests a possible relationship with cellular adhesion. This antibody detects glycoproteins of 140, 120 and 30kDa.
  • アプリケーション適用あり: IHC-Fr, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    253J bladder cancer cell line.

  • ポジティブ・コントロール
    • Kidney.
  • 特記事項


    The URO panel of antibodies was developed to immuno-anatomically dissect the nephron from glomerulus to bladder urothelium - these antibodies detect various cell surface antigens specific to segments of the renal tubule. Several have been shown to occur as tissue related markers of renal cancers. This antibody reacts predominantly with adherent cell types and is absent from cells growing in suspension, e.g. leukemia, lymphoma, lymphoblastoid, or other hematopoietic cell lines. In tissues, the URO1 antigen is expressed by glomeruli, the basal layer of transitional epithelium and skin, and the basement membrane of glands. All bladder tumors express URO1, as do most kidney tumors and other epithelial tumors (e.g. colon, ovary, lung and breast). Astrocytoma, melanoma, sarcoma, and lymphoma specimens are negative for URO1 expression.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 0.01M PBS
  • 精製度Tissue culture supernatant
  • 一次抗体 備考The URO panel of antibodies was developed to immuno-anatomically dissect the nephron from glomerulus to bladder urothelium - these antibodies detect various cell surface antigens specific to segments of the renal tubule. Several have been shown to occur as tissue related markers of renal cancers. This antibody reacts predominantly with adherent cell types and is absent from cells growing in suspension, e.g. leukemia, lymphoma, lymphoblastoid, or other hematopoietic cell lines. In tissues, the URO1 antigen is expressed by glomeruli, the basal layer of transitional epithelium and skin, and the basement membrane of glands. All bladder tumors express URO1, as do most kidney tumors and other epithelial tumors (e.g. colon, ovary, lung and breast). Astrocytoma, melanoma, sarcoma, and lymphoma specimens are negative for URO1 expression.
  • ポリ/モノモノクローナル
  • クローン名J143
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/40. Fix with acetone.
ICC 1/40.

ターゲット情報

  • 機能Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
  • 組織特異性Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
  • 配列類似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 翻訳後修飾Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA407068 antibody
    • Antigen identified by monoclonal antibody J143 antibody
    • CD49 antigen like family member C antibody
    • CD49 antigen-like family member C antibody
    • CD49c antibody
    • CD49c antigen antibody
    • FLJ34631 antibody
    • FLJ34704 antibody
    • FRP 2 antibody
    • FRP-2 antibody
    • FRP2 antibody
    • Galactoprotein B3 antibody
    • GAP B3 antibody
    • GAPB3 antibody
    • Integrin alpha 3 antibody
    • Integrin alpha-3 light chain antibody
    • Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) antibody
    • ITA3_HUMAN antibody
    • ITGA 3 antibody
    • Itga3 antibody
    • MSK18 antibody
    • VCA 2 antibody
    • VCA2 antibody
    • Very late activation protein 3 receptor alpha 3 subunit antibody
    • VL3A antibody
    • VLA 3 alpha chain antibody
    • VLA 3 subunit alpha antibody
    • VLA-3 subunit alpha antibody
    • VLA3a antibody
    see all

Anti-Integrin alpha 3 antibody [J143] 画像

  • ICC/IF image of ab11767 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11767, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Integrin alpha 3 antibody [J143] (ab11767) 使用論文

This product has been referenced in:
  • Cagnet S  et al. Signaling events mediated by a3ß1 integrin are essential for mammary tumorigenesis. Oncogene N/A:N/A (2013). Read more (PubMed: 24077284) »
  • McKeen Polizzotti L  et al. Novel Image Analysis Approach Quantifies Morphological Characteristics of 3D Breast Culture Acini with Varying Metastatic Potentials. J Biomed Biotechnol 2012:102036 (2012). ICC/IF ; Human . Read more (PubMed: 22665978) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab11767.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"