製品の概要

  • 製品名
    Anti-Integrin alpha 3 antibody [F35 177-1]
    Integrin alpha 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [F35 177-1] to Integrin alpha 3
  • アプリケーション
    適用あり: IHC-Fr, IHC-FoFrmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Cell preparation: MTSV1-7 HYE - human breast epithelial cells

  • 特記事項
    Fusion Partner: NS2

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    F35 177-1
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. Fix with acetone.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
  • 組織特異性
    Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
  • 配列類似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 翻訳後修飾
    Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA407068 antibody
    • Antigen identified by monoclonal antibody J143 antibody
    • CD49 antigen like family member C antibody
    • CD49 antigen-like family member C antibody
    • CD49c antibody
    • CD49c antigen antibody
    • FLJ34631 antibody
    • FLJ34704 antibody
    • FRP 2 antibody
    • FRP-2 antibody
    • FRP2 antibody
    • Galactoprotein B3 antibody
    • GAP B3 antibody
    • GAPB3 antibody
    • Integrin alpha 3 antibody
    • Integrin alpha-3 light chain antibody
    • Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) antibody
    • ITA3_HUMAN antibody
    • ITGA 3 antibody
    • Itga3 antibody
    • MSK18 antibody
    • VCA 2 antibody
    • VCA2 antibody
    • Very late activation protein 3 receptor alpha 3 subunit antibody
    • VL3A antibody
    • VLA 3 alpha chain antibody
    • VLA 3 subunit alpha antibody
    • VLA-3 subunit alpha antibody
    • VLA3a antibody
    see all

画像

  • Immunohistochemistry (Frozen sections) analysis of human skin tissue sections labeling Integrin alpha 3 with ab20140 at 1/1000 dilution. An undiluted goat anti-mouse polyclonal alkaline phosphatase conjugate was used as the secondary antibody.

プロトコール

参考文献

This product has been referenced in:
  • Lustosa SA  et al. Expression profiling using a cDNA array and immunohistochemistry for the extracellular matrix genes FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 in colorectal carcinoma progression and dissemination. ScientificWorldJournal 2014:102541 (2014). IHC ; Human . Read more (PubMed: 24737953) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Blocking step
Labvision Kit as blocking agent for 5 minute(s) · Concentration: 0
Username

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投稿 Nov 30 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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