Anti-Integrin alpha 2+beta 1 抗体 [P1E6] (ab78883)

製品の概要

  • 製品名Anti-Integrin alpha 2+beta 1 antibody [P1E6]
    Integrin alpha 2+beta 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [P1E6] to Integrin alpha 2+beta 1
  • アプリケーション適用あり: Flow Cyt, Inhibition Assay, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation : Human HT1080, whole cells.

  • ポジティブ・コントロール
    • Skin (basement membrane) and HepG2 cell line (IF/ICC)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration. Suitable for use with fibroblasts, epithelial cells, endothelial cells, and non activated platelets on collagen types I, III, IV, VI and laminin.
ICC/IF Use at an assay dependent concentration. Suitable for use with fresh frozen or acetone fixed tissues or cell culture.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 細胞内局在Cell Membrane; single-pass type I membrane protein
    • 参照データベース
    • 別名
      • CD29 antibody
      • CD49B antibody
      • Collagen receptor antibody
      • GPIa antibody
      • GPIIA antibody
      • Integrin alpha 2 antibody
      • Integrin beta 1 antibody
      • ITGA2 antibody
      • ITGB1 antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet membrane glycoprotein Ia antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA4 antibody
      • VLAA2 antibody
      see all

    Anti-Integrin alpha 2+beta 1 antibody [P1E6] 画像

    • ICC/IF image of ab78883 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78883, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) MCF7 cells at 5µg/ml.
    • Overlay histogram showing HT1080 cells stained with ab78883 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78883, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HT1080 cells used under the same conditions.

    Anti-Integrin alpha 2+beta 1 antibody [P1E6] (ab78883) 使用論文

    ab78883 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"